Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Glutathione found to significantly reduce acetaldehyde levels and help relieve hangovers Combination of Cysteine and Glutathione Prevents Ethanol Induced Hangover and Liver Damage by Modulation of Nrf2 Signaling in HepG2 Cells and Mice Glutathione and Hangover: The Science Backed Approach to Alcohol Recov Performance Lab